graduale, Requiem aeternam... V. In memoria by Tomás Luis de Victoria

The Choir of St John's College, Cambridge is part of the English cathedral tradition, having been founded to sing the daily liturgy in the College Chapel, though it is set apart from other English choirs of this tradition by the frequent inclusion of Continental works in its repertoire and its emphasis on polyphonic interpretations. Alongside the choir of King's College, Cambridge, it is one of the two most famous collegiate choirs in Cambridge, having had over 90 recordings published. The choir consists of fifteen Choral Scholars and twenty Choristers and Probationers, all of whom are members of St John's College, many of whom have proceeded to become distinguished musicians.

The early records of the choirs are obscure, but it is known that its origins can be traced to the original foundation of the College in 1511. The present arrangement of the choir began in 1670.

In addition to the main choir, a secondary mixed-voice choir, St John's Voices, was founded in 2013. In March 2024, it was announced that this choir would be disbanded, leaving only the primary college choir for female singers. The move was met with great controversy, leading to criticism by many notable figures.